Part:BBa_K1916009:Design

Osc7112_5903g3 + intein-CBD tag

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 589
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 636
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1156
  Illegal AgeI site found at 299
  Illegal AgeI site found at 1246
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

It is not advisable to purify this protein using metal ion affinity columns. As such the fusion protein contains an intein-CBD tag and must be purified using chitin columns.

Source

Cyanobacteria genome